REAGEN LLC
REAGEN INC, focusing on ELISA, PCR, and Lateral Flow testing kits including clinical disease detecting kits, food & feed safety test kits, and animal epidemic virus detecting kits in the US for 12
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Ofloxacin ELISA Test Kit
REAGEN™ofloxacin ELISA Test Kit enables government agencies, food manufacturers and quality assurance organizations to detect ofloxacin to as low as 0.1 ppb level in various sample types and to satisfy customer concerns about food safety.
1. The extraction of ofloxacin from meat/tissue with a high recovery rate.
2. A quick ELISA assay (less than 1.5 hours regardless of number of samples).
3. High reproducibility.Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The ofloxacin antibody has been coated in the plate wells. During the analysis, sample is added along with the ofloxacin-horseradish peroxidase (Ofloxacin-HRP) conjugate. If the ofloxacin residue is present in the sample, it will compete for the ofloxacin antibody, thereby preventing the Ofloxacin-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the ofloxacin residue concentration in the sample.
| Sample Type | Detection Limit (ng/g or ppb) |
| Meat/Liver/Kidney/Fish/Shrimp | 0.9 |
| Analytes | Cross-Reactivity (%) |
| Ofloxacin | 100 |
| Ciprofloxacin | 82 |
| Enrofloxacin | 80 |
| Danofloxacin | 42 |
| Enoxacin | 14 |
| Pipemidic acid | 11 |
| Benofloxacin | 3 |
| Oxolin acid | 4 |
| Nalidixic acid | 2 |