REAGEN LLC
REAGEN INC, focusing on ELISA, PCR, and Lateral Flow testing kits including clinical disease detecting kits, food & feed safety test kits, and animal epidemic virus detecting kits in the US for 12
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Melamine ELISA Test Kit
REAGEN™ Melamine ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of melamine in milk, yogurt, sour milk, milk powder, egg, meat, fish, feedand other food samples.
1. High recovery (>80%),rapidand cost-effective extraction methods.
2. High sensitivity(5ng/g or ppb)and low detection limit for liquid milk (25ppb).
3. A quick ELISA assay(less than 1 hours regardless of number of samples).
4. High reproducibility.
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample and HRP-Conjugated Antibody #2 are added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. At the same time, the second Antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
| Sample Type | Detection Limit (ng/g or ppb) |
| Milk | 0.025 |
| Yogurt/sour milk | 0.025 |
| Milk powder/Infant Formula | 0.2 |
| Meat /fish/Egg | 0.125 |
| Feed | 0.25 |
| Milk | 0.025 |
| Analytes | Cross-Reactivity (%) |
| Melamine | 100 |
| Cyanurate | 15 |
| Ammeline | 5 |
| Ammelide | <1 |