REAGEN LLC
REAGEN INC, focusing on ELISA, PCR, and Lateral Flow testing kits including clinical disease detecting kits, food & feed safety test kits, and animal epidemic virus detecting kits in the US for 12
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Nitrofurans ELISA Test Kit
REAGEN™Nitrofurans ELISA Test Kit enables government agencies, food manufacturers and quality assurance organizations to detect Nitrofurans to as low as 0.01 ppb level in various sample types and to satisfy customer concerns about food safety.
1. The extraction of Nitrofurans from meat/tissue with a high recovery rate.
2. A quick ELISA assay (less than 1.0 hours regardless of number of samples).
3. High reproducibility.Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The Nitrofurans antibody has been coated in the plate wells. During the analysis, sample is added along with the Nitrofurans - horseradish peroxidase (Nitrofurans-HRP) conjugate. If the Nitrofurans residue is present in the sample, it will compete for the Nitrofurans antibody, thereby preventing the Nitrofurans-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the Nitrofurans residue concentration in the sample.
| Sample Type | Detection Limit (ng/kg or ppt) |
| Meat/Liver/Kidney/Fish/Shrimp | 100 (Rapid Method) |
| milk | 100 (Rapid Method) |
| Analytes | Cross-Reactivity (%) |
| AOZ | 100 |
| AMOZ | 89 |
| AHD | 78 |
| SEM | 82 |