REAGEN LLC
REAGEN INC, focusing on ELISA, PCR, and Lateral Flow testing kits including clinical disease detecting kits, food & feed safety test kits, and animal epidemic virus detecting kits in the US for 12
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Norfloxacin ELISA Test Kit
REAGEN™ norfloxacin ELISA Test Kit enables government agencies, food manufacturers and quality assurance organizations to detect Norfloxacin in various sample types and to satisfy customer concerns about food safety.
1.The extraction of norfloxacin from feed, honey, meat, milk, tissue, serum and urine can be finished in 10 - 30 minutes with a high recovery rate of 75 - 95%.
2.A quick ELISA assay (less than 2 hours regardless of number of samples).
3.High reproducibility.
The method is based on a competitive colorimetric ELISA assay. The norfloxacin antibody has been coated in the plate wells. During the analysis, sample is added along with the norfloxacin-horseradish peroxidase (Norfloxacin-HRP) conjugate. If the norfloxacin residue is present in the sample, it will compete for the norfloxacin antibody, thereby preventing the Norfloxacin-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the Norfloxacin residue concentration in the sample.
| Sample Type | Detection Limit (ng/g or ppb) |
| Feed | 1 |
| Honey | 2 |
| Meat/Liver/Kidney/Fish/Shrimp | 1 |
| Milk | 0.5 |
| Serum | 0.5 |
| Urine | 0.5 |
| Fluoroquinolines | Cross-Reactivity (%) |
| Norfloxacin | 100 |
| Oxolinic acid | 40 |
| Pipemidic acid | 18 |
| Ofloxacin | 17 |
| Ciprofloxacin | 9 |
| Enrofloxacin | 6 |
| Flumequin | 6 |
| Cinoxacin | 1 |