REAGEN LLC
REAGEN INC, focusing on ELISA, PCR, and Lateral Flow testing kits including clinical disease detecting kits, food & feed safety test kits, and animal epidemic virus detecting kits in the US for 12
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Total Antibiotics in Milk ELISA Test Kit
REAGEN™ Total Antibiotics in Milk Test Kit provides an easy and simple test method for detecting total antibiotics in milk. Compared with current commercially available kits.
1.A quick spore growth assay (results can be read in about 3 hours).
2.Detection of wide range of antibiotics in milk.
3.High reproducibility.
4.High sensitivity and low detection limit.
The method is based on the inhibition of a standard microbial growth recommended by international organizations. The kit is presented in a microplate format where each well contains medium agar spread with bacillus spores. When the plate (or a strip of wells) is incubated at 65°C, these bacillus spores germinate and the bacillus grows so that acids are produced, which result in the pH value changes. The color of the pH indicator in the medium changes from purple to yellow. If milk samples contain antibiotics at higher concentration than the detection limit, the bacillus will not grow and therefore the medium color remains unchanged.
| Antibiotic | Detection Limit (ppb) | MRL (ppb) |
| Penicillin G | 2 | 4 |
| Ampicillin | 3 | 4 |
| Amoxicillin | 4 | 4 |
| Oxacillin | 20 | 30 |
| Cloxacillin | 30 | 30 |
| Gentamycin | 30 | 100 |
| Tylosin | 50 | 50 |
| Ceftiofur | 50 | 100 |
| Oxytetracycline | 50 | 100 |
| Tetracycline | 50 | 100 |
| Sulfamethoxazole | 200 | 500 |