REAGEN LLC
REAGEN INC, focusing on ELISA, PCR, and Lateral Flow testing kits including clinical disease detecting kits, food & feed safety test kits, and animal epidemic virus detecting kits in the US for 12
Add to Cart
Ractopamine ELISA Test Kit
REAGEN™ Ractopamine ELISA Test Kit is a competitive enzyme immunoassay for this beta-agonist drug in meat, animal tissue, milk, feed, urine, serum and plasma. Ractopamine ELISA Test Kit provides a unique extraction method, which is accurate, rapid, cost-effective and easy to use.
1.The extraction of ractopamine from feed, meat, milk, tissue and urine can be finished in 10 - 60 minutes with a high recovery rate of 80 - 120%.
2.High reproducibility.
3.A quick ELISA assay (less than 2 hours regardless of number of samples).
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
| Sample Type | Detection Limit (ng/g or ppb) |
| Feed | 0.5 |
| Meat/Liver/Kidney | 0.1 |
| Milk | 0.4 |
| Urine | 0.4 |
| Analytes | Sensitivity (ppb) | Cross-Reactivity (%) |
| Ractopamine | 0.025 | 100 |
| Ractopamine Glucuronide A | 0.06 | 51 |
| Ractopamine Glucuronide B | 0.1 | 48 |
| Ractopamine Glucuronide C | 0.03 | 95 |
| Clenbuterol | >10,000 | <0.01 |
| Colterol | >10,000 | <0.01 |
| Mabuterol | >10,000 | <0.01 |
| Isoxsuprine | 5,000 | <0.01 |
| Salmeterol | 5,000 | <0.01 |
| Fenoterol | >10,000 | <0.01 |
| Bitolterol | >10,000 | <0.01 |
| Carbuterol | >10,000 | <0.01 |
| Pirbuterol | >10,000 | <0.01 |
| Terbuterol | >10,000 | <0.01 |
| Timolol | >10,000 | <0.01 |