REAGEN LLC
REAGEN INC, focusing on ELISA, PCR, and Lateral Flow testing kits including clinical disease detecting kits, food & feed safety test kits, and animal epidemic virus detecting kits in the US for 12
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REAGEN™ Total Antibiotics in Honey Test Kit provides an easy and simple test method for detecting total antibiotics in honey. Compared with current commercially available kits,.
A quick spore growth assay (results can be read in about 3-4hours).
Detection of wide range of antibiotics in honey.
High reproducibility.
High sensitivity and low detection limit.
The method is based on the inhibition of a standard microbial growth recommended by international organizations. The kit is presented in a microplate format where each well contains medium agar spread with bacillus spores. When the plate (or a strip of wells) is incubated at 65°C, these bacillus spores germinate and the bacillus grows so that acids are produced, which result in the pH value changes. The color of the pH indicator in the medium changes from purple to yellow. If honey samples contain antibiotics at higher concentration than the detection limit, the bacillus will not grow and therefore the medium color remains unchanged.
REAGEN™Total Antibiotics in honey Test Kit has the capacity for 96 determinations for product Reference #: RND99043-01 and 288 determinations for product Reference #: RND99043-02, including negative and positive controls. Return any unused plates/strips/wells to the plastic bag provided in the original package and reseal them. The shelf life is 12 months when the kit is properly stored.
| Kit Contents | Amount | Storage |
| Microtiter Plate | 96-well plates (8 wells x 12 strips) | 2-8°C |
| Negative Control | 1.5 mL | -20°C |
| Positive Control | 1.5 mL | -20°C |
| Plate Seal | 2 sheets | NA |
| 10X Honey Diluent | 15 mL | 2-8°C |
| QC Certificate | 1 | NA |
| Kit Contents | Amount | Storage |
| Microtiter Plate | 3x96-well plates (8wells x 12 strips) | 2-8°C |
| Negative Control | 3 x 1.5 mL | -20°C |
| Positive Control | 3 x 1.5 mL | -20°C |
| Plate Seal | 6 sheets | NA |
| 10X Honey Diluent | 30 mL | 2-8°C |
| QC Certificate | 1 | NA |
* I Upon arrival, immediately store the honey Positive Control at -20°C. See storage temperature for other components. After reconstitution of the negative and positive controls, aliquot 0.1mL into separate tubes and store in freezer at -20°C.
| Antibiotic | Detection Limit (ppb) |
| Penicillin G | 12 |
| Ampicillin | 18 |
| Amoxicillin | 18 |
| Oxacillin | 90 |
| Cloxacillin | 180 |
| Tylosin | 300 |
| Ceftiofur | 300 |
| Oxytetracycline | 300 |
| Tetracycline | 300 |
| Gentamycin | 150 |