REAGEN LLC
REAGEN INC, focusing on ELISA, PCR, and Lateral Flow testing kits including clinical disease detecting kits, food & feed safety test kits, and animal epidemic virus detecting kits in the US for 12
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Diethylstilbestrol ELISA Test Kit Manual
REAGEN™ Diethylstilbestrol ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Diethylstilbestrol in feed, fish, meat, milk, milk powder, serum/plasma, shrimp, tissue and urine.
1.High recovery (80%-105%),rapid, cost-effective extraction methods.
2.High sensitivity(0.15 ng/g or ppb) and low detection limit (meat/fish/shrimp 0.075 ppb and urine 0.25ppb).
3.A quick ELISA assay (less than 2 hours regardless of number of samples).
4.High reproducibility.
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
| Sample Type | Detection Limit (ng/g or ppb) |
| Meat/fish/Shrimp | 0.075 |
| Urine | 0.25 |
| Feed | 0.15 |
| Milk | 0.2 |
| Milk power | 2 |
| Serum/Plasma | 0.75 |
| Analytes | Cross-Reactivity (%) |
| Diethylstilbestrol | 100 |
| DES-glucuronide | 90 |
| Hexestrol | 35 |
| Dienestrol | 10 |