REAGEN LLC
REAGEN INC, focusing on ELISA, PCR, and Lateral Flow testing kits including clinical disease detecting kits, food & feed safety test kits, and animal epidemic virus detecting kits in the US for 12
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Vitamin B7 (Biotin) Test Kit
REAGEN™Vitamin B7 Test Kit is quantitative analysis of total Vitamin B7 in food ,feed and drug.
1. rapid, and cost-effective extraction methods,the same as other Vitamin.
2. The kit provide positive control and serial standards
3. High sensitivity (0.04μg/100g)
4. High recovery(90%-105%)
5. High reproducibility
REAGEN™Sample and Vitamin B7 culture medium add to the 96-microwell which has the Lactobacillus plantarum subsp. lactis (leichmannii),if the sample hasVitamin B7 the Lactobacillus plantarum will grow,after culture 37℃,44-48h, read the OD by microplate reader 610 - 630 nm (or540 - 550 nm),The sample of the turbidity compared with standard curve can get Biotin content.
REAGEN™ Vitamin B7 Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8℃ .The shelf life is 6 months when the kit is properly stored.