REAGEN LLC
REAGEN INC, focusing on ELISA, PCR, and Lateral Flow testing kits including clinical disease detecting kits, food & feed safety test kits, and animal epidemic virus detecting kits in the US for 12
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Okadaic Acid(DSP)ELISA Test Kit
REAGEN™Okadaic Acid (DSP) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Okadaic Acid (DSP) in mussels and water samples.
1.High recovery (80-105%), rapid (10-30 minutes), and cost-effective extraction methods
2.High sensitivity (0.3 ng/g or ppb) and low detection limit (30 ppb for mussel samples and 6 ppb for water samples).
3.High reproducibility.
4.A quick ELISA assay(less than 1.5 hours regardless of number of samples).
The method is based on a competitive colorimetric ELISA assay. The second antibody against Anti-Okadaic Acid Antibody was coated in the plate wells. During the analysis, sample is added along with the okadaic acid-horseradish peroxidase conjugate (Okadaic Acid-HRP Conjugate) and Anti- Okadaic Acid Antibody. If the okadaic acid residue is present in the sample, it will compete with Okadaic Acid-HRP Conjugate for the Anti-Okadaic Acid Antibody, thereby preventing the Okadaic Acid-HRP Conjugate from binding to the Anti-Okadaic Acid Antibody. The Anti-Okadaic Acid Antibody is then bound by the second antibody immobilized on the plate. After a washing step and addition of the TMB substrate solution, a color signal is produced. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The resulting color intensity has an inverse relationship with the okadaic acid residue concentration in the sample.
| Sample Type | Detection Limit (ng/g or ppb) |
| Mussels | 30 |
| Water | 6 |
| Analytes | Cross-Reactivity (%) |
| Okadaic Acid | 100.0 |
| DTX1 | 40 |
| DTX2 | 46 |
| Neosaxitoxin | < 0.01 |
| Saxitoxin | < 0.01 |
| YTX | < 0.01 |