REAGEN LLC
REAGEN INC, focusing on ELISA, PCR, and Lateral Flow testing kits including clinical disease detecting kits, food & feed safety test kits, and animal epidemic virus detecting kits in the US for 12
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REAGEN™Fumonisin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Fumonisin in cereals, dried meat/fish, feed, milk, seed and oils.
1. High recovery (80-120%),rapid(10-20minutes), cost-effective extraction methods.
2. A quick ELISA assay (less than 1 hours regardless of number of samples).
3. High reproducibility.
4. High sensitivity (1 ng/g or ppb).
The method is based on a competitive colorimetric ELISA assay. The toxin of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target toxin and enzyme-conjugate. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the toxin attached to the well. The enzyme-conjugate, will bined with the primary antibody that is complexed to the toxin coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
| Sample Type | Detection Limit (ng/g or ppb) |
| Meat/fish/sereal/feed/seed | 100 |
| Milk | 10 |
| Oils | 10 |
| Analytes | Cross-Reactivity (%) |
| Fumonisin B1 | 100.0 |
| Fumonisin B2 | 94 |
| Fumonisin B3 | 145 |
| T-2 | <0.1 |
| DON | <0.1 |